Service descriptions and rates

If you are interested in leveraging Next Gen sequencing power but the services listed don’t exactly match what you want to do, please contact us . We’re happy to discuss custom sequencing projects including protocol development.

Turnaround time for our services is dependent on type of service and the number of samples that you submit. Our prices are based on multiplexing many samples on each run, therefore if you submit a few samples they won’t be processed till the run is filled. If you submit full runs of samples (95+ samples) for microbiome sequencing, they are usually completed in 4-8 weeks depending on our current queue. If your project is different enough from our usual process that we deem it “protocol development”, we won’t be able to estimate turn around but will have regular conversations updating you on the progress. Please contact us for current turn around time estimates.

Users that are more concerned with turnaround time than cost, please talk to us. It is often possible for your samples to be sequenced quickly on less than full runs, you would just need to pay the balance of the run cost.

If you are shipping samples to us, please consult our shipping guide and/or contact us with any shipping questions.

Microbiome and Amplicon Analysis

MARS offers several on-ramps for this analysis. Full service sample processing is available by combining DNA extraction with our DNA to data service which includes quantification, library prep, and sequencing at least 10,000 sequences per sample. We don’t have a hard cutoff for quantity or quality of DNA, but be aware the very dilute or impure DNA may lead to difficulty amplifying and biased community recovered. If you submit DNA, we recommend at least 50ng in 20ul. We can also sequence user generated libraries provided that they can use either MARS or Illumina standard sequencing primers (Client Amplicon library to data). Users who either want to control all of the library prep or who are investigating multiple targets, may want to use the 2-step Amplicon to data approach where the user does the initial PCR then we attach the indexes in a second PCR. If you are interested in either Amplicon library to data or 2-step Amplicon to data, please contact us *before* you do any library prep.

graphical overview of amplicon services

Service Internal Rate External nonprofit Rate
DNA Isolation $12/sample $17/sample
DNA to Data $24/sample $33/sample
2-step Amplicon to Data $25/sample $44/sample
Client Amplicon library to Data $18/sample $25/sample
OTU clustering, mothur standard process $50/batch $70/batch

If you would like us to cluster your data into OTUs we will use a tailored variation of this mothur processing script. The data returned will be OTU tables and alpha/beta diversity calculated for 3% (species), 5% (genus), 10% (family/order?), and maybe even 15%(phyla/subphyla??). The 15% is a maybe because it depends on the data if I’ll be able to cluster that high. I’ll also taxonomically ID the OTUs to genus because that’s as far as I think you can reliably ID 16S v4 data. There are data pipelines that will return species level names but I believe that says more about what sequences are in the database than what sequences are in your data. Please talk to me if you think your dataset would be appropriate for species level identification and we can decide together a course of action.
Additional custom data analysis is available including multivariate statistical analyses and publication quality figure generation, contact us for a quote.

Genome Analysis
For BSL1 isolates, you can give us a cell pellet for DNA extraction before DNA to Genome processing. We also accept DNA or user generated libraries with no BSL restriction. These prices are for NexteraXT library prep which works for many but not all bacterial genomes (high GC organisms don’t work well with this kit). If you’re not sure what library prep your genome will need contact us to discuss your project.

Service Internal Rate External nonprofit Rate
DNA to Genome $155/sample $215/sample
Client library to Genome $120/sample $165/sample

Consultation and Education

Service Internal Rate External nonprofit Rate
Consulting and custom data analysis $65/hour $90/hour
2 day workshop $300/participant $400/participant
5 day workshop $500/participant $675/participant

Instrument Use

MARS instrumentation can be accessed either unassisted by authorize users or with the assistance of MARS personnel. Once a user has been trained on an instrument, fill out the Equipment request form to reserve time. Instrument access is billed per whole hour. Aliquots of many of the reagents and kits that MARS uses can be purchased for cost +5% aliquot fee. Contact us to find out if we have a particular reagent or kit.

Instrument Assisted Rate Unassisted Rate
MiSeq $161/run $121/run
EpMotion 5075 $50/hour $20/hour
QIAgility $50/hour $20/hour
Synergy Plate Reader $35/hour $5/hour
Qubit $35/hour $5/hour
QIAxcel Consumables and machine time for fastDNA are $6.50/12 sample set